bool HitPolicy::compareByScoreTStart(const PacBio::BAM::BamRecord & a, const PacBio::BAM::BamRecord & b) const {
assert(a.Impl().HasTag(AS) and b.Impl().HasTag(AS));
assert(a.FullName() == b.FullName());
const int aScore = a.Impl().TagValue(AS).ToInt32();
const int bScore = b.Impl().TagValue(AS).ToInt32();
if (aScore == bScore)
return a.ReferenceStart() < b.ReferenceEnd();
return Score(aScore, _sign).WorseThan(Score(bScore, _sign));
}
bool FilterCriteria::Satisfy(const PacBio::BAM::BamRecord& record) const
{
assert(record.IsMapped() and record.Impl().HasTag(AS));
DNALength alnLength = static_cast<DNALength>(
record.Sequence(PacBio::BAM::Orientation::NATIVE, true, true).size());
PacBio::BAM::Cigar cigar = record.CigarData();
uint32_t nMatch = 0, nMismatch = 0, nIns = 0, nDel = 0;
for (auto op : cigar) {
uint32_t n = op.Length();
switch (op.Type()) {
case PacBio::BAM::CigarOperationType::SEQUENCE_MATCH:
nMatch += n;
break;
case PacBio::BAM::CigarOperationType::SEQUENCE_MISMATCH:
nMismatch += n;
break;
case PacBio::BAM::CigarOperationType::ALIGNMENT_MATCH:
nMismatch += n;
break;
case PacBio::BAM::CigarOperationType::INSERTION:
nIns += n;
break;
case PacBio::BAM::CigarOperationType::DELETION:
nDel += n;
break;
default:
break;
}
}
//TODO: Use = X instead of M in blasr.
float pctSimilarity = 100 * nMatch / static_cast<float>(alnLength);
float pctAccuracy = 100 * nMatch / static_cast<float>(nMismatch + nMatch + nIns + nDel);
Score s(static_cast<float>(record.Impl().TagValue("AS").ToInt32()), ScoreSign::NEGATIVE);
return Satisfy(alnLength, pctSimilarity, pctAccuracy, s);
}
void FASTASequence::Copy(const PacBio::BAM::BamRecord & record) {
FASTASequence::Copy(record.Impl().Name(), record.Sequence());
}