//**********************************************************************************************************************
int GetSeqsCommand::readCount(){
	try {
		string thisOutputDir = outputDir;
		if (outputDir == "") {  thisOutputDir += m->hasPath(countfile);  }
		map<string, string> variables; 
		variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile));
        variables["[extension]"] = m->getExtension(countfile);
		string outputFileName = getOutputFileName("count", variables);
		
		ofstream out;
		m->openOutputFile(outputFileName, out);
		
		ifstream in;
		m->openInputFile(countfile, in);
		
		bool wroteSomething = false;
		int selectedCount = 0;
		
        string headers = m->getline(in); m->gobble(in);
        out << headers << endl;
        string test = headers; vector<string> pieces = m->splitWhiteSpace(test);
        
        string name, rest; int thisTotal; rest = "";
        while (!in.eof()) {
            
            if (m->control_pressed) { in.close();  out.close();  m->mothurRemove(outputFileName);  return 0; }
            
            in >> name; m->gobble(in); 
            in >> thisTotal; m->gobble(in);
            if (pieces.size() > 2) {  rest = m->getline(in); m->gobble(in);  }
            if (m->debug) { m->mothurOut("[DEBUG]: " + name + '\t' + rest + "\n"); }
            
            if (names.count(name) != 0) {
                out << name << '\t' << thisTotal << '\t' << rest << endl;
                wroteSomething = true;
                selectedCount+= thisTotal;
            }
        }
        in.close();
		out.close();
        
        //check for groups that have been eliminated
        CountTable ct;
        if (ct.testGroups(outputFileName)) {
            ct.readTable(outputFileName, true, false);
            ct.printTable(outputFileName);
        }
		
		if (wroteSomething == false) {  m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
		outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
		
		m->mothurOut("Selected " + toString(selectedCount) + " sequences from your count file."); m->mothurOutEndLine();
        
		return 0;
	}
	catch(exception& e) {
		m->errorOut(e, "GetSeqsCommand", "readCount");
		exit(1);
	}
}
//**********************************************************************************************************************
ClassifyOtuCommand::ClassifyOtuCommand(string option)  {
	try{
		abort = false; calledHelp = false;   
		allLines = 1;
		labels.clear();
				
		//allow user to run help
		if (option == "help") { 
			help(); abort = true; calledHelp = true;
		}else if(option == "citation") { citation(); abort = true; calledHelp = true;} 
		else {
			vector<string> myArray = setParameters();
			
			OptionParser parser(option);
			map<string, string> parameters = parser.getParameters();
			
			ValidParameters validParameter;
			map<string, string>::iterator it;
		
			//check to make sure all parameters are valid for command
			for (it = parameters.begin(); it != parameters.end(); it++) { 
				if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
			}
			
			//initialize outputTypes
			vector<string> tempOutNames;
			outputTypes["constaxonomy"] = tempOutNames;
			outputTypes["taxsummary"] = tempOutNames;
		
			//if the user changes the input directory command factory will send this info to us in the output parameter 
			string inputDir = validParameter.validFile(parameters, "inputdir", false);		
			if (inputDir == "not found"){	inputDir = "";		}
			else {
				string path;
				it = parameters.find("list");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["list"] = inputDir + it->second;		}
				}
				
				it = parameters.find("name");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["name"] = inputDir + it->second;		}
				}
				
				it = parameters.find("taxonomy");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["taxonomy"] = inputDir + it->second;		}
				}
				
				it = parameters.find("reftaxonomy");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["reftaxonomy"] = inputDir + it->second;		}
				}
				
				it = parameters.find("group");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["group"] = inputDir + it->second;		}
				}
                
                it = parameters.find("count");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["count"] = inputDir + it->second;		}
				}
			}

			
			//if the user changes the output directory command factory will send this info to us in the output parameter 
			outputDir = validParameter.validFile(parameters, "outputdir", false);		if (outputDir == "not found"){	outputDir = "";		}
			
			//check for required parameters
			listfile = validParameter.validFile(parameters, "list", true);
			if (listfile == "not found") {				
				//if there is a current list file, use it
				listfile = m->getListFile(); 
				if (listfile != "") {  m->mothurOut("Using " + listfile + " as input file for the list parameter."); m->mothurOutEndLine(); }
				else { 	m->mothurOut("You have no current listfile and the list parameter is required."); m->mothurOutEndLine(); abort = true; }
			}
			else if (listfile == "not open") { abort = true; }	
			else { m->setListFile(listfile); }
			
			taxfile = validParameter.validFile(parameters, "taxonomy", true);
			if (taxfile == "not found") {  //if there is a current list file, use it
				taxfile = m->getTaxonomyFile(); 
				if (taxfile != "") {  m->mothurOut("Using " + taxfile + " as input file for the taxonomy parameter."); m->mothurOutEndLine(); }
				else { 	m->mothurOut("You have no current taxonomy file and the taxonomy parameter is required."); m->mothurOutEndLine(); abort = true; }
			}
			else if (taxfile == "not open") { abort = true; }
			else { m->setTaxonomyFile(taxfile); }
			
			refTaxonomy = validParameter.validFile(parameters, "reftaxonomy", true);
			if (refTaxonomy == "not found") { refTaxonomy = ""; m->mothurOut("reftaxonomy is not required, but if given will keep the rankIDs in the summary file static."); m->mothurOutEndLine(); }
			else if (refTaxonomy == "not open") { abort = true; }
	
			namefile = validParameter.validFile(parameters, "name", true);
			if (namefile == "not open") { namefile = ""; abort = true; }	
			else if (namefile == "not found") { namefile = ""; }
			else { m->setNameFile(namefile); }
			
			groupfile = validParameter.validFile(parameters, "group", true);
			if (groupfile == "not open") { abort = true; }	
			else if (groupfile == "not found") { groupfile = ""; }
			else { m->setGroupFile(groupfile); }
            
            countfile = validParameter.validFile(parameters, "count", true);
			if (countfile == "not open") { countfile = ""; abort = true; }
			else if (countfile == "not found") { countfile = "";  }	
			else { m->setCountTableFile(countfile); }
            
            if ((namefile != "") && (countfile != "")) {
                m->mothurOut("[ERROR]: you may only use one of the following: name or count."); m->mothurOutEndLine(); abort = true;
            }
			
            if ((groupfile != "") && (countfile != "")) {
                m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true;
            }

			
			//check for optional parameter and set defaults
			// ...at some point should added some additional type checking...
			label = validParameter.validFile(parameters, "label", false);			
			if (label == "not found") { label = ""; allLines = 1;  }
			else { 
				if(label != "all") {  m->splitAtDash(label, labels);  allLines = 0;  }
				else { allLines = 1;  }
			}
            
			basis = validParameter.validFile(parameters, "basis", false);
			if (basis == "not found") { basis = "otu"; }	
			
			if ((basis != "otu") && (basis != "sequence")) { m->mothurOut("Invalid option for basis. basis options are otu and sequence, using otu."); m->mothurOutEndLine(); }
			
			string temp = validParameter.validFile(parameters, "cutoff", false);			if (temp == "not found") { temp = "51"; }
			m->mothurConvert(temp, cutoff); 
			
			temp = validParameter.validFile(parameters, "probs", false);					if (temp == "not found"){	temp = "true";			}
			probs = m->isTrue(temp);
            
            temp = validParameter.validFile(parameters, "persample", false);		if (temp == "not found"){	temp = "f";		}
			persample = m->isTrue(temp);
			
            if ((groupfile == "") && (countfile == "")) { if (persample) { m->mothurOut("persample is only valid with a group file, or count file with group information. Setting persample=f.\n"); persample = false; } 
            }
            if (countfile != "") {
                CountTable cts;
                if (!cts.testGroups(countfile)) { 
                    if (persample) { m->mothurOut("persample is only valid with a group file, or count file with group information. Setting persample=f.\n"); persample = false; }
                }
            }
			
			if ((cutoff < 51) || (cutoff > 100)) { m->mothurOut("cutoff must be above 50, and no greater than 100."); m->mothurOutEndLine(); abort = true;  }
			
            if (countfile == "") {
                if (namefile == ""){
                    vector<string> files; files.push_back(taxfile);
                    parser.getNameFile(files);
                }
            }
			
		}
	}
	catch(exception& e) {
		m->errorOut(e, "ClassifyOtuCommand", "ClassifyOtuCommand");
		exit(1);
	}
}
Beispiel #3
0
int MergeGroupsCommand::processCountFile(DesignMap*& designMap){
    try {
        CountTable countTable;
        if (!countTable.testGroups(countfile)) { m->mothurOut("[ERROR]: your countfile contains no group information, please correct.\n"); m->setControl_pressed(true); return 0; }
        
        //read countTable
        countTable.readTable(countfile, true, false);
        
        //fill Groups - checks for "all" and for any typo groups
        vector<string> nameGroups = countTable.getNamesOfGroups();
        if (Groups.size() == 0) { Groups = nameGroups; }
        
        
        vector<string> dnamesGroups = designMap->getNamesGroups();
        
        //sanity check
        bool error = false;
        if (nameGroups.size() == dnamesGroups.size()) { //at least there are the same number
            //is every group in counttable also in designmap
            for (int i = 0; i < nameGroups.size(); i++) {
                if (m->getControl_pressed()) { break; }
                if (!util.inUsersGroups(nameGroups[i], dnamesGroups)) { error = true; break; }
            }
            
        }
        if (error) { m->mothurOut("[ERROR]: Your countfile does not contain the same groups as your design file, please correct\n"); m->setControl_pressed(true); return 0; }
        
        //user selected groups - remove some groups from table
        if (Groups.size() != nameGroups.size()) {
            for (int i = 0; i < nameGroups.size(); i++) {
                if (!util.inUsersGroups(nameGroups[i], Groups)) { countTable.removeGroup(nameGroups[i]); }
            }
        }
        //ask again in case order changed
        nameGroups = countTable.getNamesOfGroups();
        int numGroups = nameGroups.size();
        
        //create new table
        CountTable newTable;
        vector<string> treatments = designMap->getCategory();
        map<string, vector<int> > clearedMap;
        for (int i = 0; i < treatments.size(); i++) {
            newTable.addGroup(treatments[i]);
            vector<int> temp;
            clearedMap[treatments[i]] = temp;
        }
        treatments = newTable.getNamesOfGroups();
        
        set<string> namesToRemove;
        vector<string> namesOfSeqs = countTable.getNamesOfSeqs();
        for (int i = 0; i < namesOfSeqs.size(); i++) {
            
            if (m->getControl_pressed()) { break; }
            
            vector<int> thisSeqsCounts = countTable.getGroupCounts(namesOfSeqs[i]);
            map<string, vector<int> > thisSeqsMap = clearedMap;
            
            for (int j = 0; j < numGroups; j++) {
                thisSeqsMap[designMap->get(nameGroups[j])].push_back(thisSeqsCounts[j]);
            }
        
            //create new counts for seq for new table
            vector<int> newCounts; int totalAbund = 0;
            for (int j = 0; j < treatments.size(); j++){
                int abund = mergeAbund(thisSeqsMap[treatments[j]]);
                newCounts.push_back(abund);  //order matters, add in count for each treatment in new table.
                totalAbund += abund;
            }
            
            //add seq to new table
            if(totalAbund == 0) {
                namesToRemove.insert(namesOfSeqs[i]);
            }else { newTable.push_back(namesOfSeqs[i], newCounts); }
        }
        
        if (error) { m->setControl_pressed(true); return 0; }
        
        //remove sequences zeroed out by median method
        if (namesToRemove.size() != 0) {
            //print names
            ofstream out;
            string accnosFile = "accnosFile.temp";
            util.openOutputFile(accnosFile, out);
            
            //output to .accnos file
            for (set<string>::iterator it = namesToRemove.begin(); it != namesToRemove.end(); it++) {
                if (m->getControl_pressed()) {  out.close(); util.mothurRemove(accnosFile); return 0; }
                out << *it << endl;
            }
            out.close();

            //run remove.seqs
            string inputString = "accnos=" + accnosFile + ", fasta=" + fastafile;
            
            m->mothurOut("/******************************************/"); m->mothurOutEndLine();
            m->mothurOut("Running command: remove.seqs(" + inputString + ")"); m->mothurOutEndLine();
            current->setMothurCalling(true);
            
            Command* removeCommand = new RemoveSeqsCommand(inputString);
            removeCommand->execute();
            
            map<string, vector<string> > filenames = removeCommand->getOutputFiles();
            
            delete removeCommand;
            current->setMothurCalling(false);
            m->mothurOut("/******************************************/"); m->mothurOutEndLine();
            
            util.mothurRemove(accnosFile);
        }
    
        string thisOutputDir = outputDir;
        if (outputDir == "") {  thisOutputDir += util.hasPath(countfile);  }
        map<string, string> variables;
        variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(countfile));
        variables["[extension]"] = util.getExtension(countfile);
        string outputFileName = getOutputFileName("count", variables);
        outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
        
        newTable.printTable(outputFileName);
        
        return 0;
        
    }
    catch(exception& e) {
        m->errorOut(e, "MergeGroupsCommand", "processCountFile");
        exit(1);
    }
}
Beispiel #4
0
//**********************************************************************************************************************
SharedCommand::SharedCommand(string option)  {
	try {
        abort = false; calledHelp = false; pickedGroups=false;
		allLines = 1;

		//allow user to run help
		if(option == "help") { help(); abort = true; calledHelp = true; }
		else if(option == "citation") { citation(); abort = true; calledHelp = true;}

		else {

			 vector<string> myArray = setParameters();

			 OptionParser parser(option);
			 map<string, string> parameters = parser.getParameters();

			 ValidParameters validParameter;
			 map<string, string>::iterator it;

			 //check to make sure all parameters are valid for command
			 for (it = parameters.begin(); it != parameters.end(); it++) {
				 if (!validParameter.isValidParameter(it->first, myArray, it->second)) {  abort = true;  }
			 }

			 //if the user changes the input directory command factory will send this info to us in the output parameter
			string inputDir = validParameter.valid(parameters, "inputdir");
			 if (inputDir == "not found"){	inputDir = "";		}
			 else {
				 string path;
				 it = parameters.find("list");
				 //user has given a template file
				 if(it != parameters.end()){
					 path = util.hasPath(it->second);
					 //if the user has not given a path then, add inputdir. else leave path alone.
					 if (path == "") {	parameters["list"] = inputDir + it->second;		}
				 }

				 it = parameters.find("group");
				 //user has given a template file
				 if(it != parameters.end()){
					 path = util.hasPath(it->second);
					 //if the user has not given a path then, add inputdir. else leave path alone.
					 if (path == "") {	parameters["group"] = inputDir + it->second;		}
				 }

				 it = parameters.find("count");
				 //user has given a template file
				 if(it != parameters.end()){
					 path = util.hasPath(it->second);
					 //if the user has not given a path then, add inputdir. else leave path alone.
					 if (path == "") {	parameters["count"] = inputDir + it->second;		}
				 }

                 it = parameters.find("biom");
				 //user has given a template file
				 if(it != parameters.end()){
					 path = util.hasPath(it->second);
					 //if the user has not given a path then, add inputdir. else leave path alone.
					 if (path == "") {	parameters["biom"] = inputDir + it->second;		}
				 }
			 }

             vector<string> tempOutNames;
             outputTypes["shared"] = tempOutNames;
             outputTypes["group"] = tempOutNames;
             outputTypes["map"] = tempOutNames;

			 //if the user changes the output directory command factory will send this info to us in the output parameter
			 outputDir = validParameter.valid(parameters, "outputdir");		if (outputDir == "not found"){	outputDir = "";	}

			 //check for required parameters
			 listfile = validParameter.validFile(parameters, "list");
			 if (listfile == "not open") { listfile = ""; abort = true; }
			 else if (listfile == "not found") { listfile = "";  }
			 else { current->setListFile(listfile); }

             biomfile = validParameter.validFile(parameters, "biom");
             if (biomfile == "not open") { biomfile = ""; abort = true; }
             else if (biomfile == "not found") { biomfile = "";  }
             else { current->setBiomFile(biomfile); }

			 ordergroupfile = validParameter.validFile(parameters, "ordergroup");
			 if (ordergroupfile == "not open") { abort = true; }
			 else if (ordergroupfile == "not found") { ordergroupfile = ""; }

			 groupfile = validParameter.validFile(parameters, "group");
			 if (groupfile == "not open") { groupfile = ""; abort = true; }
			 else if (groupfile == "not found") { groupfile = ""; }
			 else {  current->setGroupFile(groupfile); }

             countfile = validParameter.validFile(parameters, "count");
             if (countfile == "not open") { countfile = ""; abort = true; }
             else if (countfile == "not found") { countfile = ""; }
             else {
                 current->setCountFile(countfile);
                 CountTable temp;
                 if (!temp.testGroups(countfile)) {
                     m->mothurOut("\n[WARNING]: Your count file does not have group info, all reads will be assigned to mothurGroup.\n");
                     
                     temp.readTable(countfile, false, false); //dont read groups
                     map<string, int> seqs = temp.getNameMap();
                     
                     CountTable newCountTable;
                     newCountTable.addGroup("mothurGroup");
                     
                     for (map<string, int>::iterator it = seqs.begin(); it != seqs.end(); it++) {
                         vector<int> counts; counts.push_back(it->second);
                         newCountTable.push_back(it->first, counts);
                     }
                     
                     string newCountfileName = util.getRootName(countfile) + "mothurGroup" + util.getExtension(countfile);
                     newCountTable.printTable(newCountfileName);
                     
                     current->setCountFile(newCountfileName);
                     countfile = newCountfileName;
                     outputNames.push_back(newCountfileName);
                 }
             }

            if ((biomfile == "") && (listfile == "") && (countfile == "")) { //you must provide at least one of the following
				//is there are current file available for either of these?
				//give priority to list, then biom, then count
				listfile = current->getListFile();
				if (listfile != "") {  m->mothurOut("Using " + listfile + " as input file for the list parameter.\n");  }
				else {
					biomfile = current->getBiomFile();
                    if (biomfile != "") {  m->mothurOut("Using " + biomfile + " as input file for the biom parameter.\n"); }
					else {
                        countfile = current->getCountFile();
                        if (countfile != "") {  m->mothurOut("Using " + countfile + " as input file for the count parameter.\n");  }
                        else {
                            m->mothurOut("[ERROR]: No valid current files. You must provide a list or biom or count file before you can use the make.shared command.\n");  abort = true;
                        }

					}
				}
			}
			else if ((biomfile != "") && (listfile != "")) { m->mothurOut("When executing a make.shared command you must enter ONLY ONE of the following: list or biom.\n"); abort = true; }

			if (listfile != "") {
				if ((groupfile == "") && (countfile == "")) {
					groupfile = current->getGroupFile();
					if (groupfile != "") {  m->mothurOut("Using " + groupfile + " as input file for the group parameter.\n");  }
					else {
						countfile = current->getCountFile();
                        if (countfile != "") {  m->mothurOut("Using " + countfile + " as input file for the count parameter.\n"); }
                        else { m->mothurOut("[ERROR]: You need to provide a groupfile or countfile if you are going to use the list format.\n");  abort = true; }
					}
				}
			}


			 string groups = validParameter.valid(parameters, "groups");
			 if (groups == "not found") { groups = ""; }
			 else {
                 pickedGroups=true;
				 util.splitAtDash(groups, Groups);
                if (Groups.size() != 0) { if (Groups[0]== "all") { Groups.clear(); } }
			 }

			 //check for optional parameter and set defaults
			 // ...at some point should added some additional type checking...
			 string label = validParameter.valid(parameters, "label");
			 if (label == "not found") { label = ""; }
			 else {
				 if(label != "all") {  util.splitAtDash(label, labels);  allLines = 0;  }
				 else { allLines = 1;  }
			 }
            
            if ((listfile == "") && (biomfile == "") && (countfile != "")) { //building a shared file from a count file, require label
                if (labels.size() == 0) {
                    m->mothurOut("[ERROR]: You must provide a label when converting a count file to a shared file, please correct.\n");  abort = true;
                }
            }
		}

	}
	catch(exception& e) {
		m->errorOut(e, "SharedCommand", "SharedCommand");
		exit(1);
	}
}