Exemple #1
0
Int2 Main (void)

{
    Char buf[256] = { '\0' };  /* Used below for name and version. */
    Int2 status = 0;    /* return value of function. */

    StringCpy(buf, "bl2seq ");
    StringNCat(buf, BlastGetVersionNumber(), sizeof(buf)-StringLen(buf)-1);
    if (! GetArgs (buf, NUMARG, myargs)) {
        return (1);
    }

    UseLocalAsnloadDataAndErrMsg ();

    if (! SeqEntryLoad())
                return 1;

    ErrSetMessageLevel(SEV_WARNING);

    if (myargs[ARG_FORCE_OLD].intvalue != 0)
        status = Main_old();
    else
        status = Main_new();

    FreeArgs(NUMARG, myargs);

    return status;
}
Exemple #2
0
Int2 Nlm_Main(void) {
    Boolean use_new_engine=FALSE;
    char buf[256] = { '\0' };

    StringCpy(buf, "mgblast ");
    StringNCat(buf, BlastGetVersionNumber(), sizeof(buf)-StringLen(buf)-1);
    if (! GetArgs (buf, NUMARG, myargs))
       return (1);

    UseLocalAsnloadDataAndErrMsg ();

    if (! SeqEntryLoad())
        return 1;

    ErrSetMessageLevel(SEV_WARNING);
    /*
    if (myargs[ARG_FORCE_OLD].intvalue == 0 &&
                  myargs[ARG_OUTTYPE].intvalue > 1 &&
                      myargs[ARG_GILIST].strvalue == NULL)
          use_new_engine = readdb_use_new_blast(myargs[ARG_DB].strvalue);
    if (myargs[ARG_MASKEDQUERY].strvalue)
        use_new_engine = FALSE;
    */
    
    /*if (use_new_engine)
        return Main_new();
    else */
        return Main_old();
}
Exemple #3
0
Int2 Main(void)
{
	AsnIoPtr aip;
	SeqEntryPtr sep;
	BytePtr buf;
	Uint2 size = 32000, count;
	AsnIoMemPtr aimp;

					/* check command line arguments */

	if ( ! GetArgs("testmem",NUMARG, myargs))
		return 1;

					/* load the sequence alphabets  */
					/* (and sequence parse trees)   */
	if (! SeqEntryLoad())
		ErrShow();

					/* open the ASN.1 input file in the right mode */

	if ((aip = AsnIoOpen (myargs[0].strvalue, myargs[1].intvalue?"rb":"r"))
          == NULL)
		ErrShow();
	sep = SeqEntryAsnRead(aip, NULL);   	/* read the entry */
	AsnIoClose(aip);

	SeqEntryPack(sep);						/* pack it */

	buf = MemNew(size);                     /* allocate a buffer */
	aimp = AsnIoMemOpen("wb", buf, size);	/* open to write asn1 to it */
	SeqEntryAsnWrite(sep, aimp->aip, NULL);	/* write it */
	AsnIoFlush(aimp->aip);					/* flush it */
	count = aimp->count;					/* record how many bytes in it */
	AsnIoMemClose(aimp);					/* close it */

	SeqEntryFree(sep);						/* release the object */

	aimp = AsnIoMemOpen("rb", buf, count);	/* open to read from buffer */
	sep = SeqEntryAsnRead(aimp->aip, NULL);	/* read it */
	AsnIoMemClose(aimp);					/* close it */
	MemFree(buf);							/* free the buffer */

				  				/* open the output file */

	aip = AsnIoOpen (myargs[2].strvalue, "w");
	SeqEntryAsnWrite(sep, aip, NULL);	/* print it */
	AsnIoClose(aip);

	SeqEntryFree(sep);

	return(0);
}
Exemple #4
0
/*****************************************************************************
*
*   Main program loop to read, process, write SeqEntrys
*
*****************************************************************************/
Int2 Main(void)
{
    AsnIoPtr aipout=NULL, aipin;
    AsnTypePtr atp, atp_inst;
    AsnModulePtr amp;
    DataVal dv;
    CharPtr ftype;
    BioseqPtr bsp;

    /* check command line arguments */

    if ( ! GetArgs("asn2xml 1.0",NUMARG, myargs))
        return 1;

    /* load the sequence alphabets  */
    /* (and sequence parse trees)   */
    if (! SeqEntryLoad())
        ErrShow();
    if (! SubmitAsnLoad())
        ErrShow();
    if (! SeqCodeSetLoad())
        ErrShow();
    /* get pointer to all loaded ASN.1 modules */
    amp = AsnAllModPtr();
    if (amp == NULL)
    {
        ErrShow();
        return 1;
    }

    if (myargs[1].intvalue)
        atp = AsnFind("Seq-entry");
    else if (myargs[2].intvalue)
        atp = AsnFind("Seq-submit");
    else
        atp = AsnFind("Bioseq-set");    /* get the initial type pointers */
    if (atp == NULL)
    {
        ErrShow();
        return 1;
    }

    atp_inst = AsnFind("Bioseq.inst");
    if (atp_inst == NULL)
    {
        ErrShow();
        return 1;
    }

    /* open the ASN.1 input file in the right mode */

    if ((aipin = AsnIoOpen (myargs[0].strvalue, myargs[3].intvalue?"rb":"r"))
            == NULL)
    {
        ErrPostEx(SEV_ERROR,0,0, "Can't open %s", myargs[0].strvalue);
        ErrShow();
        return 1;
    }

    /* open the ASN.1 output file in the right mode */

    if (myargs[4].strvalue != NULL)   /* output desired? */
    {
        ftype = "wx";

        if ((aipout = AsnIoOpen (myargs[4].strvalue, ftype)) == NULL)
        {
            ErrPostEx(SEV_ERROR,0,0, "Can't open %s", myargs[4].strvalue);
            ErrShow();
            return 1;
        }
    }

    /* log errors instead of die */
    if (myargs[5].strvalue != NULL)
    {
        if (! ErrSetLog (myargs[5].strvalue))
        {
            ErrShow();
            return 1;
        }
        else
            ErrSetOpts (ERR_CONTINUE, ERR_LOG_ON);
    }

    while ((atp = AsnReadId(aipin, amp, atp)) != NULL)
    {
        if (atp == atp_inst)    /* need object loader convert */
        {
            bsp = BioseqNew();  /* need newly initialized bsp */
            BioseqInstAsnRead(bsp, aipin, atp);
            BioseqInstAsnWrite(bsp, aipout, atp);
            bsp = BioseqFree(bsp);
        }
        else
        {
            AsnReadVal(aipin, atp, &dv); /* read it */
            AsnWrite(aipout, atp, &dv);	 /* write it */
            AsnKillValue(atp, &dv);      /* free it */
        }
    }

    AsnIoClose(aipin);
    AsnIoClose(aipout);

    return(0);
}
Exemple #5
0
Int2 Main(void)
{
	AsnIoPtr aip;
	FILE * aa = NULL, * na = NULL, * ql = NULL;
	SeqEntryPtr sep;
	SeqSubmitPtr ssp;
	AsnTypePtr atp, atp2;
	AsnModulePtr amp;
	Uint1 group_segs = 0;
	Boolean limit_to_genbank,
		make_dna,
		make_protein,
		make_quality,
		far_quality,
		do_it;
	

					/* check command line arguments */

	if ( ! GetArgs("asn2fast",NUMARG, myargs))
		return 1;

					/* load the sequence alphabets  */
					/* (and sequence parse trees)   */
	if (! SeqEntryLoad())
	{
		ErrShow();
		return 1;
	}
				    /* get pointer to all loaded ASN.1 modules */
	amp = AsnAllModPtr();
	if (amp == NULL)
	{
		ErrShow();
		return 1;
	}

	if (myargs[11].intvalue) {
		if (! SubmitAsnLoad())
			Message(MSG_FATAL, "Unable to load parse trees.");
		
		atp2 = AsnFind("Seq-submit");
		if (atp2 == NULL)
			Message(MSG_FATAL, "Unable to find Seq-submit");
		atp = AsnFind("Seq-submit");
		if (atp == NULL)
			Message(MSG_FATAL, "Unable to find Seq-submit");

	} else {
		atp = AsnFind("Bioseq-set"); /* get the initial type pointers */
		if (atp == NULL)
		{
			ErrShow();
			return 1;
		}
	
		atp2 = AsnFind("Bioseq-set.seq-set.E");
		if (atp2 == NULL)
		{
			ErrShow();
			return 1;
		}
	}

	make_protein = (Boolean)(myargs[7].intvalue);
	make_dna = (Boolean)(myargs[8].intvalue);
	make_quality = (Boolean)(myargs[12].intvalue);
	far_quality = (Boolean)(myargs[14].intvalue);

					/* open the ASN.1 input file in the right mode */

	if ((aip = AsnIoOpen (myargs[0].strvalue, myargs[2].intvalue?"rb":"r"))
          == NULL)
	{
		ErrShow();
		return 1;
	}

				  				/* open the output file */

	if ((myargs[3].strvalue != NULL) && (make_protein))
	{
		if ( (aa = FileOpen (myargs[3].strvalue, "w")) == NULL)
		{
			ErrShow();
			return 1;
		}
	}

	if ((myargs[4].strvalue != NULL) && (make_dna))
	{
		if ( (na = FileOpen (myargs[4].strvalue, "w")) == NULL)
		{
			ErrShow();
			return 1;
		}
	}

	if ((myargs[13].strvalue != NULL) && (make_quality))
	{
		if ( (ql = FileOpen (myargs[13].strvalue, "w")) == NULL)
		{
			ErrShow();
			return 1;
		}
	}

                                /* log errors instead of die */
    if (myargs[5].strvalue != NULL)
    {
        if (! ErrSetLog (myargs[5].strvalue))
            ErrShow();
        else
            ErrSetOpts (ERR_CONTINUE, ERR_LOG_ON);
   }

	if (myargs[6].intvalue)  /* combine segmented seqs */
	{
		group_segs = 1;
		if (myargs[10].intvalue)
			group_segs = 3;       /* and instantiate virtuals */
	}

	limit_to_genbank = (Boolean)(myargs[9].intvalue);

	if (myargs [15].intvalue) {
		ID1BioseqFetchEnable ("asn2fast", FALSE);
	}
	if (myargs [16].intvalue) {
		LocalSeqFetchInit (FALSE);
	}

	if ( myargs[1].intvalue)   /* read one Seq-entry */
	{

		sep = SeqEntryAsnRead(aip, NULL);
		do_it = TRUE;
		if (limit_to_genbank)
			do_it = CheckIsGenBank(sep);
		if (do_it)
		{
			if (make_protein)
				SeqEntrysToFasta(sep, aa, FALSE, group_segs);
			if (make_dna)
				SeqEntrysToFasta(sep, na, TRUE, group_segs);
			if (make_quality) {
				if (far_quality) {
					SeqEntryExplore (sep, (Pointer) ql, PrintFarQualScores);
				} else {
					SeqEntryExplore (sep, (Pointer) ql, PrintQualScores);
				}
			}
		}
		SeqEntryFree(sep);
	}
	else if ( myargs[11].intvalue)   /* read Seq-submit's */
	{
		while ((atp = AsnReadId(aip, amp, atp)) != NULL)
		{
			if (atp == atp2)    /* top level Seq-entry */
			{
				ssp = SeqSubmitAsnRead(aip, atp);
				if (ssp->datatype == 1)
				{
					sep = (SeqEntryPtr) ssp->data;
					do_it = TRUE;
					if (limit_to_genbank)
						do_it = CheckIsGenBank(sep);
					if (do_it)
					{
						if (make_protein)
							SeqEntrysToFasta(sep, aa, FALSE, group_segs);
						if (make_dna)
							SeqEntrysToFasta(sep, na, TRUE, group_segs);
						if (make_quality) {
							if (far_quality) {
								SeqEntryExplore (sep, (Pointer) ql, PrintFarQualScores);
							} else {
								SeqEntryExplore (sep, (Pointer) ql, PrintQualScores);
							}
						}
					}
				}
				SeqSubmitFree(ssp);
			}
			else
			{
				AsnReadVal(aip, atp, NULL);
			}
		}
	}
	else                      /* read Seq-entry's from a Bioseq-set */
	{
		while ((atp = AsnReadId(aip, amp, atp)) != NULL)
		{
			if (atp == atp2)    /* top level Seq-entry */
			{
				sep = SeqEntryAsnRead(aip, atp);
				do_it = TRUE;
				if (limit_to_genbank)
					do_it = CheckIsGenBank(sep);
				if (do_it)
				{
					if (make_protein)
						SeqEntrysToFasta(sep, aa, FALSE, group_segs);
					if (make_dna)
						SeqEntrysToFasta(sep, na, TRUE, group_segs);
					if (make_quality) {
						if (far_quality) {
							SeqEntryExplore (sep, (Pointer) ql, PrintFarQualScores);
						} else {
							SeqEntryExplore (sep, (Pointer) ql, PrintQualScores);
						}
					}
				}
				SeqEntryFree(sep);
			}
			else
			{
				AsnReadVal(aip, atp, NULL);
			}
		}
	}

	AsnIoClose(aip);
	if (make_protein)
		FileClose(aa);
	if (make_dna)
		FileClose(na);
	if (make_quality)
		FileClose (ql);

	if (myargs [16].intvalue) {
		LocalSeqFetchDisable ();
	}
	if (myargs [15].intvalue) {
		ID1BioseqFetchDisable ();
	}

	return(0);
}
Exemple #6
0
/*****************************************************************************
*
*   This is the main program.
*     It reads command line or initial dialogue arguments
*     It initializes parse trees, entrez
*     It makes a list of the relevant files to search
*     It then calls ProcessFileList to open each file and process it
*
*****************************************************************************/
Int2 Main (void)
{
  Char         div [8];
  FileListPtr  flp = NULL;
  FILE         *fp;
  Int2         i;
  Boolean      is_network, did_init=FALSE;
  FileListPtr  next;
  CharPtr      outputfile;

  if (! GetArgs ("CdScan", NUMARGS, myargs))   /* get input args */
	goto ret;

											   /* process input args */
  if (myargs[0].intvalue == 1)
  	is_na = TRUE;      /* scan nucleic acids */
  else
  	is_na = FALSE;     /* scan proteins */
  format = (Int2)(myargs[1].intvalue);
  is_custom = (Boolean)(myargs[2].intvalue);
  if (myargs[3].intvalue)   /* show progress */
  {
  	pmon = MonitorStrNew("CdScan", 40);
  }
  outputfile = myargs[4].strvalue;   /* output file name */

  if (pmon != NULL)
  	MonitorStrValue(pmon, "Reading Parse Trees");

  if (! SeqEntryLoad() || ! SubmitAsnLoad())   /* read ASN.1 parse trees */
  {
  	Message(MSG_ERROR, "Can't open parse trees");
  	goto ret;
  }

  if (! PrintTemplateSetLoad ("asn2ff.prt"))
  {
    Message(MSG_ERROR, "Can't load print templates");
	goto ret;
  }

  if (pmon != NULL)
  	MonitorStrValue(pmon, "Initializing Entrez");

  if (! EntrezInit ("cdscan", FALSE, &is_network)) /* init Entrez */
  {
  	Message(MSG_ERROR, "Can't initialize Entrez");
  	goto ret;
  }

  did_init = TRUE;
  if (is_network)
  {
      Message (MSG_ERROR, "Network service does not allow scanning");
  	goto ret;
  }

  if (pmon != NULL)
  	MonitorStrValue(pmon, "Building File List");

  eip = EntrezGetInfo ();                        /* set up the file lists */
  if ((eip == NULL) || (eip->div_info == NULL))
  {
  	Message(MSG_ERROR, "Can't find Entrez file info");
  	goto ret;
  }


  flp = FileListNew (NULL, INT2_MIN, NULL, NULL);
  if (flp == NULL)
  {
  	Message(MSG_ERROR, "Can't allocate file list");
  	goto ret;
  }

  div_info = eip->div_info;
  for (i = 0; i < eip->div_count; i++)
  {
  	StringNCpy (div, div_info [i].tag, sizeof (div) - 1);
  	if (! is_na)
  	{
      	CdEnumFiles (CdDir_rec, TYP_AA, div, EnumerateFiles, &flp);
      }
  	else
  	{
      	CdEnumFiles (CdDir_rec, TYP_NT, div, EnumerateFiles, &flp);
  	}
  }

  fp = FileOpen (outputfile, "w");   /* test that we can open output file */
  if (fp == NULL)
  {
  	Message(MSG_ERROR, "Can't open [%s]", outputfile);
  	goto ret;
  }

  FileClose (fp);     /* will be reopened for each input file */

  ProcessFileList (flp, outputfile);              /* process the file list */

ret:                                                         /* clean up */

  if (pmon != NULL)     /* close the progress monitor */
	MonitorFree(pmon);

  if (did_init)
	  EntrezFini();         /* close entrez */

  while (flp != NULL)       /* free file list */
  {
    next = flp->next;
    MemFree (flp->fdir);
    MemFree (flp->fname);
    MemFree (flp);
    flp = next;
  }

  return 0;
}
Exemple #7
0
Int2 Main(void)
{
    WWWInfoPtr info;
    WWWErrorCode error;
    BEDataPtr pBdata;
    Int4 count;
    Uint4 *ids;
    Char tmp[512];

    if((error = WWWReadPosting(&info)) != WWWErrOk) {
        ErrPostEx(SEV_FATAL, 88, 0, "Error in processing WWW request");
        return 1;
    }

    if(WWWGetMethod(info) == COMMAND_LINE) {
        WWWInfoFree(info);
        
        if((pBdata = BEMakeCLParam()) == NULL)
            return 1;
        
    } else {
        
        if(!ErrSetLogfile (QSRV_LOGFILE_STD_NAME, ELOG_APPEND))
            return 1;
        
        if((pBdata = BEMakeWWWParam(info)) == NULL) {
            printf("Content-type: text/html\n\n");
            printf("QSRV_STATUS 802 Invalid input parameters <PRE><BR>\n");
            fflush(stdout);
            
            ErrPostEx(SEV_ERROR, 88, 0, 
                      "Error in reading parameters. "
                      "Please check, that query string was set\n");
            return 1;
        }
    }

    if(!pBdata->commandline) {
        if(pBdata->savetodisk) 
            printf("Content-type: chemical/seq-%s-genbank\n\n", 
                   pBdata->database == 0 ? "na" : "aa");
        else {
            printf("Content-type: text/html\n\n");
            printf("<HTML><HEADER><TITLE>Batch Entrez results"
                   "</TITLE></HEADER><PRE>\n");
        }
    }

    if (! ID1BioseqFetchEnable("Nbatch", TRUE)) {
        printf("Cannot initialize ID1\n");
        return 1;
    }

    if(pBdata->html) {
        init_www(); /* initializing WWW mode */
        head_tail_ff(NULL, BatchHead, BatchTail);
      
    }

    SeqEntryLoad();

    switch(pBdata->request_type) {
    case REQ_DEFAULT:
        if(pBdata->uids == NULL) {
            count = BEGetUidsFromQuery(pBdata->query, &ids, 
                               pBdata->database == 0, /* Nucleotide ? */
                               FALSE);
        } else {
            count = BE_ReadIds(pBdata, &ids);
        }
        break;
    case REQ_ADVANCED_QUERY:
    case REQ_ORGANISM:
        count = BEGetUidsFromQuery(pBdata->query, &ids, 
                                   pBdata->database == 0, /* Nucleotide ? */
                                   FALSE);
        break;
    case REQ_LIST_OF_GIS:
        count = BE_ReadIds(pBdata, &ids);
        break;    
    }
    pBdata->count = count;
    
    QSRVWriteLogInfo(pBdata);
    
    if(count < 0) {
        if(pBdata->commandline)
            ErrPostEx(SEV_ERROR, 0, count, "Error in searching the database");
        else
            printf("QSRV_STATUS %d Error in searching database\n", count);
        return 1;
    }
    
    if(count == 0) {
        if(pBdata->commandline)
            ErrPostEx(SEV_INFO, 0,0, "No entries found");
        else
            printf("QSRV_STATUS 901 OK No entries found\n", count);
        return 1;
    }
    
    if((count > 20000 && pBdata->format == F_GEN) ||
       (count > 100000 && pBdata->format != F_GILIST)) {

        if(pBdata->commandline)
            ErrPostEx(SEV_WARNING, 0,0, "Number of sequences %d exceed limit", 
                      count);
        else
            printf("QSRV_STATUS 803 Error Number of sequences %d "
                   "exceed limit\n", count);
        
        pBdata->format = F_GILIST;
    }

    if(!pBdata->noheader && !pBdata->commandline) 
        printf("QSRV_STATUS 900 OK: %d entr%s found <PRE><BR>\n", 
               count, count == 1? "y" : "ies");

    fflush(stdout);
    
    /* Printing results */
    
    BEPrintIds(pBdata, ids, count);
    
    /* Clearing memory */

    MemFree(ids);
    BEFreeCLParam(pBdata);

    return 0;
}
Exemple #8
0
char *readASNSeq(const short whichEntry, const char *filename,
                const long skiplines,
                const short format,     /* note: this is kASNseqentry or kASNseqset */
                long *seqlen, short *nseq,
                short *error, char **seqid )
{
  AsnIoPtr aip = NULL;
  SeqEntryPtr the_set;
  AsnTypePtr atp, atp2;
  AsnModulePtr amp;
  Boolean inIsBinary= FALSE; /* damn, why can't asn routines test this? */
  char  *seq, stemp[200];
  int   i, count;

  *seqlen= 0;
  *nseq= 0;
  *error= 0;
  seq= NULL;

/*fprintf(stderr,"readASNseq: SeqEntryLoad\n");*/
    /* asn dictionary setups */
  if (! SeqEntryLoad()) goto errxit; /*  sequence alphabets (and sequence parse trees) */
  amp = AsnAllModPtr();   /* get pointer to all loaded ASN.1 modules */
  if (amp == NULL) goto errxit;
  atp = AsnFind("Bioseq-set");    /* get the initial type pointers */
  if (atp == NULL) goto errxit;
  atp2 = AsnFind("Bioseq-set.seq-set.E");
  if (atp2 == NULL) goto errxit;

      /* open the ASN.1 input file in the right mode */
/*fprintf(stderr,"readASNseq: AsnIoOpen(%s)\n", filename);*/
  if ((aip = AsnIoOpen(filename, inIsBinary?"rb":"r")) == NULL) goto errxit;
  for (i=0; i<skiplines; i++) fgets( stemp, 255, aip->fp);  /* this may mess up asn routines... */

  if (! ErrSetLog ("stderr"))  goto errxit;
  else ErrSetOpts(ERR_CONTINUE, ERR_LOG_ON);    /*??  log errors instead of die */

  seq= NULL;
  if (format == kASNseqentry) {  /* read one Seq-entry */
/*fprintf(stderr,"readASNseq: SeqEntryAsnRead\n");*/
    the_set = SeqEntryAsnRead(aip, NULL);
    SeqEntryToRaw(the_set, false, whichEntry, nseq, &seq, seqid, seqlen);
    SeqEntryFree(the_set);
    goto goodexit;
    }

  else   {                   /* read Seq-entry's from a Bioseq-set */
    count = 0;
/*fprintf(stderr,"readASNseq: AsnReadId\n");*/
    while ((atp = AsnReadId(aip, amp, atp)) != NULL) {
      if (atp == atp2)  {  /* top level Seq-entry */
        the_set = SeqEntryAsnRead(aip, atp);
        SeqEntryToRaw(the_set, false, whichEntry, nseq, &seq, seqid, seqlen);
        SeqEntryFree(the_set);
        if (*nseq >= whichEntry) goto goodexit;
        }
      else
        AsnReadVal(aip, atp, NULL);
      count++;
      }
    }

goodexit:
  AsnIoClose(aip);
  *error= 0;
  return seq;

errxit:
  AsnIoClose(aip);
  *error= eASNerr;
  if (seq) free(seq);
  return NULL;
}
Exemple #9
0
Int2 Main(void)
{
	AsnIoPtr       aip;
	SeqEntryPtr    sep;
	BioseqPtr PNTR seqlist;
	Int4           seqnum, i, numseg, lens[10], j;
	Int2           ctr;
	SeqPortPtr     spp;
	Uint1          residue;
	FILE*          fp;
	CharPtr        title;
	Char           buffer[101];
	MonitorPtr     mon;

						/* check command line arguments */

	if ( ! GetArgs("SeqTest",NUMARG, myargs))
		return 1;

	mon = MonitorStrNew("SeqTest", 40);
	SetProgMon(StdProgMon, (Pointer)mon);

	/*
	** Load SeqEntry object loader and sequence alphabets
	*/

	if (! SeqEntryLoad()) {
		Message(MSG_ERROR, "SeqEntryLoad failed");
		return 1;
	}

	/*
	** Use the file "example.prt" as the ASN I/O stream.  This file
	** can be found in the ncbi/demo.  It is in ASN.1 Print Value format.
	*/

	if ((aip = AsnIoOpen(myargs[0].strvalue, "r")) == NULL)
		return 1;

	/*
	** Write the output to "seqtest.out".
	*/

	fp = FileOpen(myargs[1].strvalue, "w");
	fprintf(fp, "Sequence summary:\n\n");

	/*
	** Read in the whole entry into the Sequence Entry Pointer, sep.
	** Close the ASN stream, which in turn closes the input file.
	*/

	sep = SeqEntryAsnRead(aip, NULL);
	aip = AsnIoClose(aip);

	mon = MonitorFree(mon);
	SetProgMon(NULL, NULL);

	/*
	** Determine how many Bioseqs are in this SeqEntry. Allocate
	** enough memory to hold a list of pointer to all of these
	** Bioseqs.  Invoke an Explore function to "visit"each Bioseq.
	** We are allowed to pass one pointer for use by the exploring
	** function, in this case, "BuildList".
	*/

	seqnum = BioseqCount(sep);
	seqlist = MemNew((size_t)(seqnum * sizeof(BioseqPtr)));
	BioseqExplore(sep, (Pointer) seqlist, BuildList);

	/*
	** For each Bioseq in the SeqEntry write out it's title
	** len, number of gaps, and number of segments. Write out
	** the length of each segment, up to 10.
	*/

	for(i = 0; i < seqnum; i++) {
		numseg = BioseqCountSegs(seqlist[i]);
		title = BioseqGetTitle(seqlist[i]);
		FilePuts((VoidPtr)title, fp);
		FilePuts("\n", fp);
		fprintf(fp, "len=%ld gaps=%ld segs=%ld\n", BioseqGetLen(seqlist[i]),
		BioseqGetGaps(seqlist[i]), numseg);
		if ((numseg > 1) && (numseg <= 10)) {
			BioseqGetSegLens (seqlist[i], lens);
			for (j = 0; j < numseg; j++)
				fprintf(fp, "  len = %ld\n", lens[j]);
		}
		FilePuts("\n", fp);
	}

	spp = SeqPortNew(seqlist[0], 0, -1, 0, Seq_code_iupacna);
	if (spp == NULL)
		Message(MSG_ERROR, "fail on SeqPortNew");

	fprintf(fp, "SeqPort: plus strand with SeqPortGetResidue\n\n");

	i = 0;
	while ((residue = SeqPortGetResidue(spp)) != SEQPORT_EOF) {
		if (! IS_residue(residue)) {
			buffer[i] = '\0';
			fprintf(fp, "%s\n", buffer);
			i = 0;
			switch (residue)
			{
				case SEQPORT_VIRT:
					fprintf(fp, "[Gap]\n");
					break;
				case SEQPORT_EOS:
					fprintf(fp, "[EOS]\n");
					break;
				default:
					fprintf(fp, "[Invalid Residue]\n");
					break;
			}

		}
		else {
			buffer[i] = residue;
			i++;
			if (i == 60) {
				buffer[i] = '\0';
				fprintf(fp, "%s\n", buffer);
				i = 0;
			}
		}
	}

	if (i) {
		buffer[i] = '\0';
		fprintf(fp, "%s\n", buffer);
	}

	fprintf(fp, "[EOF]\n");
	SeqPortFree(spp);

	fprintf(fp, "\nSeqPort on minus with SeqPortRead\n\n");
	spp = SeqPortNew(seqlist[0], 0, -1, Seq_strand_minus, Seq_code_iupacna);

	if (spp == NULL)
		Message(MSG_ERROR, "fail on SeqPortNew");

	do {
		ctr = SeqPortRead(spp, (Uint1Ptr)buffer, 60);
		if (ctr > 0) {
			buffer[ctr] = '\0';
			fprintf(fp, "%s\n", buffer);
		} else {
			ctr *= -1;
			switch (ctr)
			{
				case SEQPORT_VIRT:
					fprintf(fp, "[Gap]\n");
					break;
				case SEQPORT_EOS:
					fprintf(fp, "[EOS]\n");
					break;
				case SEQPORT_EOF:
					fprintf(fp, "[EOF]\n");
					break;
				default:
					fprintf(fp, "[Invalid Residue]\n");
					break;
			}
		}
	} while (ctr != SEQPORT_EOF);

	SeqPortFree(spp);

	/*
	** Write out the nucleic acid sequences in this SeqEntry
	*/

	fprintf(fp, "\nNucleic Acids in FASTA format:\n\n");
	SeqEntryToFasta(sep, fp, TRUE);

	/*
	** Write out the protein sequences in this SeqEntry.
	*/

	fprintf(fp, "\nProteins in FASTA format:\n\n");
	SeqEntryToFasta(sep, fp, FALSE);

	/*
	** Close the output file and free up allocated space.
	*/

	fclose(fp);
	MemFree(seqlist);
	SeqEntryFree(sep);

	return 0;
}