int gt_runidxlocali(const IdxlocaliOptions *idxlocalioptions,GtError *err)
{
Genericindex *genericindex = NULL;
bool haserr = false;
GtLogger *logger;
const GtEncseq *encseq = NULL;
logger = gt_logger_new(idxlocalioptions->verbose,
GT_LOGGER_DEFLT_PREFIX, stdout);
if (idxlocalioptions->doonline)
{
GtEncseqLoader *el;
el = gt_encseq_loader_new();
gt_encseq_loader_require_multiseq_support(el);
gt_encseq_loader_drop_description_support(el);
gt_encseq_loader_set_logger(el, logger);
encseq = gt_encseq_loader_load(el, gt_str_get(idxlocalioptions->indexname),
err);
gt_encseq_loader_delete(el);
if (encseq == NULL)
{
haserr = true;
}
} else
{
genericindex = genericindex_new(gt_str_get(idxlocalioptions->indexname),
idxlocalioptions->withesa,
idxlocalioptions->withesa ||
idxlocalioptions->docompare,
false,
true,
0,
logger,
err);
if (genericindex == NULL)
{
haserr = true;
} else
{
encseq = genericindex_getencseq(genericindex);
}
}
if (!haserr)
{
GtSeqIterator *seqit;
const GtUchar *query;
unsigned long querylen;
char *desc = NULL;
int retval;
Limdfsresources *limdfsresources = NULL;
const AbstractDfstransformer *dfst;
SWdpresource *swdpresource = NULL;
Showmatchinfo showmatchinfo;
ProcessIdxMatch processmatch;
GtAlphabet *a;
void *processmatchinfoonline, *processmatchinfooffline;
Storematchinfo storeonline, storeoffline;
a = gt_encseq_alphabet(encseq);
if (idxlocalioptions->docompare)
{
processmatch = storematch;
gt_initstorematch(&storeonline,encseq);
gt_initstorematch(&storeoffline,encseq);
processmatchinfoonline = &storeonline;
processmatchinfooffline = &storeoffline;
} else
{
processmatch = showmatch;
showmatchinfo.encseq = encseq;
showmatchinfo.characters = gt_alphabet_characters(a);
showmatchinfo.wildcardshow = gt_alphabet_wildcard_show(a);
showmatchinfo.showalignment = idxlocalioptions->showalignment;
processmatchinfoonline = processmatchinfooffline = &showmatchinfo;
}
if (idxlocalioptions->doonline || idxlocalioptions->docompare)
{
swdpresource = gt_newSWdpresource(idxlocalioptions->matchscore,
idxlocalioptions->mismatchscore,
idxlocalioptions->gapextend,
idxlocalioptions->threshold,
idxlocalioptions->showalignment,
processmatch,
processmatchinfoonline);
}
dfst = gt_locali_AbstractDfstransformer();
if (!idxlocalioptions->doonline || idxlocalioptions->docompare)
{
gt_assert(genericindex != NULL);
limdfsresources = gt_newLimdfsresources(genericindex,
true,
0,
0, /* maxpathlength */
true, /* keepexpandedonstack */
processmatch,
processmatchinfooffline,
NULL, /* processresult */
NULL, /* processresult info */
dfst);
}
seqit = gt_seq_iterator_sequence_buffer_new(idxlocalioptions->queryfiles,
err);
if (!seqit)
haserr = true;
if (!haserr)
{
gt_seq_iterator_set_symbolmap(seqit, gt_alphabet_symbolmap(a));
for (showmatchinfo.queryunit = 0; /* Nothing */;
showmatchinfo.queryunit++)
{
retval = gt_seq_iterator_next(seqit,
&query,
&querylen,
&desc,
err);
if (retval < 0)
{
haserr = true;
break;
}
if (retval == 0)
{
break;
}
printf("process sequence " Formatuint64_t " of length %lu\n",
PRINTuint64_tcast(showmatchinfo.queryunit),querylen);
if (idxlocalioptions->doonline || idxlocalioptions->docompare)
{
gt_multiapplysmithwaterman(swdpresource,encseq,query,querylen);
}
if (!idxlocalioptions->doonline || idxlocalioptions->docompare)
{
gt_indexbasedlocali(limdfsresources,
idxlocalioptions->matchscore,
idxlocalioptions->mismatchscore,
idxlocalioptions->gapstart,
idxlocalioptions->gapextend,
idxlocalioptions->threshold,
query,
querylen,
dfst);
}
if (idxlocalioptions->docompare)
{
gt_checkandresetstorematch(showmatchinfo.queryunit,
&storeonline,&storeoffline);
}
}
if (limdfsresources != NULL)
{
gt_freeLimdfsresources(&limdfsresources,dfst);
}
if (swdpresource != NULL)
{
gt_freeSWdpresource(swdpresource);
swdpresource = NULL;
}
gt_seq_iterator_delete(seqit);
}
if (idxlocalioptions->docompare)
{
gt_freestorematch(&storeonline);
gt_freestorematch(&storeoffline);
}
}
if (genericindex == NULL)
{
gt_encseq_delete((GtEncseq *) encseq);
encseq = NULL;
} else
{
genericindex_delete(genericindex);
}
gt_logger_delete(logger);
logger = NULL;
return haserr ? -1 : 0;
}
int gt_genomediff_pck_shu_simple(GtLogger *logger,
const GtGenomediffArguments *arguments,
GtError *err)
{
int had_err = 0;
int retval;
GtSeqIterator *queries = NULL;
const GtUchar *symbolmap, *currentQuery;
const GtAlphabet *alphabet;
GtUchar c_sym = 0,
g_sym = 0;
uint64_t queryNo;
char *description = NULL;
unsigned long queryLength,
subjectLength = 0,
currentSuffix;
double avgShuLength,
currentShuLength = 0.0,
/*gc_subject,*/
gc_query /*, gc*/;
const FMindex *subjectindex = NULL;
Genericindex *genericindexSubject;
const GtEncseq *encseq = NULL;
double *ln_n_fac;
/* get the precalculation of ln(n!) for 0<n<max_ln_n_fac */
ln_n_fac = gt_get_ln_n_fac(arguments->max_ln_n_fac);
gt_log_log("ln(max_ln_n_fac!) = %f\n",
ln_n_fac[arguments->max_ln_n_fac]);
genericindexSubject = genericindex_new(gt_str_get(
arguments->indexname),
arguments->with_esa,
true,
false,
true,
arguments->user_max_depth,
logger,
err);
if (genericindexSubject == NULL)
{
had_err = 1;
}
else
{
encseq = genericindex_getencseq(genericindexSubject);
}
if (!had_err)
{
subjectLength = genericindex_get_totallength(genericindexSubject) - 1;
/*subjectLength /= 2;*/
/*gt_log_log("subject length: %lu", subjectLength);*/
subjectindex = genericindex_get_packedindex(genericindexSubject);
queries = gt_seqiterator_sequence_buffer_new(
arguments->queryname,
err);
gt_assert(queries);
alphabet = gt_encseq_alphabet(encseq);
/* makes assumption that alphabet is dna, it has to calculate the gc! */
if (!gt_alphabet_is_dna(alphabet))
{
fprintf(stderr, "error: Sequences need to be dna");
had_err = 1;
}
else
{
symbolmap = gt_alphabet_symbolmap(alphabet);
gt_seqiterator_set_symbolmap(queries, symbolmap);
c_sym = gt_alphabet_encode(alphabet, 'c');
g_sym = gt_alphabet_encode(alphabet, 'g');
}
}
for (queryNo = 0; !had_err; queryNo++)
{
retval = gt_seqiterator_next(queries,
¤tQuery,
&queryLength,
&description,
err);
if ( retval != 1)
{
if (retval < 0)
{
gt_free(description);
}
break;
}
gt_logger_log(logger,
"found query of length: %lu",
queryLength);
avgShuLength = 0.0;
gc_query = 0.0;
for (currentSuffix = 0; currentSuffix < queryLength; currentSuffix++)
{
currentShuLength = (double) gt_pck_getShuStringLength(
subjectindex,
¤tQuery[currentSuffix],
queryLength - currentSuffix);
avgShuLength += currentShuLength;
if (currentQuery[currentSuffix] == c_sym ||
currentQuery[currentSuffix] == g_sym)
{
gc_query++;
}
}
if (arguments->shulen_only)
{
printf("# Query %d sum of shulen:\n %.0f\n",
(int) queryNo, avgShuLength);
}
else
{
avgShuLength /= (double) queryLength;
gc_query /= (double) queryLength;
gt_logger_log(logger, "Query %d has an average SHUstring length "
"of\n# shulength: %f",
(int) queryNo, avgShuLength);
gt_logger_log(logger, "Query description: %s", description);
gt_log_log("Query (i): %s", description);
/* XXX Fehlerabfragen einbauen */
if ( !had_err )
{
double div, kr;
gt_logger_log(logger, "shulen:\n%f", avgShuLength);
gt_log_log("shu: %f, gc: %f, len: %lu",
avgShuLength, gc_query, subjectLength);
div = gt_divergence(arguments->divergence_rel_err,
arguments->divergence_abs_err,
arguments->divergence_m,
arguments->divergence_threshold,
avgShuLength,
subjectLength,
gc_query,
ln_n_fac,
arguments->max_ln_n_fac);
gt_logger_log(logger, "divergence:\n%f", div);
kr = gt_calculateKr(div);
printf("# Kr:\n%f\n", kr);
}
}
}
gt_free(ln_n_fac);
gt_seqiterator_delete(queries);
genericindex_delete(genericindexSubject);
return had_err;
}
int runidxlocali(const IdxlocaliOptions *idxlocalioptions,GtError *err)
{
Genericindex *genericindex = NULL;
bool haserr = false;
Verboseinfo *verboseinfo;
const Encodedsequence *encseq = NULL;
verboseinfo = newverboseinfo(idxlocalioptions->verbose);
if (idxlocalioptions->doonline)
{
encseq = mapencodedsequence (true,
idxlocalioptions->indexname,
true,
false,
false,
true,
verboseinfo,
err);
if (encseq == NULL)
{
haserr = true;
}
} else
{
genericindex = genericindex_new(idxlocalioptions->indexname,
idxlocalioptions->withesa,
idxlocalioptions->withesa ||
idxlocalioptions->docompare,
false,
true,
0,
verboseinfo,
err);
if (genericindex == NULL)
{
haserr = true;
} else
{
encseq = genericindex_getencseq(genericindex);
}
}
if (!haserr)
{
GtSeqIterator *seqit;
const GtUchar *query;
unsigned long querylen;
char *desc = NULL;
int retval;
Limdfsresources *limdfsresources = NULL;
const AbstractDfstransformer *dfst;
SWdpresource *swdpresource = NULL;
Showmatchinfo showmatchinfo;
Processmatch processmatch;
void *processmatchinfoonline, *processmatchinfooffline;
Storematchinfo storeonline, storeoffline;
if (idxlocalioptions->docompare)
{
processmatch = storematch;
initstorematch(&storeonline,encseq);
initstorematch(&storeoffline,encseq);
processmatchinfoonline = &storeonline;
processmatchinfooffline = &storeoffline;
} else
{
processmatch = showmatch;
showmatchinfo.encseq = encseq;
showmatchinfo.characters = getencseqAlphabetcharacters(encseq);
showmatchinfo.wildcardshow = getencseqAlphabetwildcardshow(encseq);
showmatchinfo.showalignment = idxlocalioptions->showalignment;
processmatchinfoonline = processmatchinfooffline = &showmatchinfo;
}
if (idxlocalioptions->doonline || idxlocalioptions->docompare)
{
swdpresource = newSWdpresource(idxlocalioptions->matchscore,
idxlocalioptions->mismatchscore,
idxlocalioptions->gapextend,
idxlocalioptions->threshold,
idxlocalioptions->showalignment,
processmatch,
processmatchinfoonline);
}
dfst = locali_AbstractDfstransformer();
if (!idxlocalioptions->doonline || idxlocalioptions->docompare)
{
gt_assert(genericindex != NULL);
limdfsresources = newLimdfsresources(genericindex,
true,
0,
0, /* maxpathlength */
true, /* keepexpandedonstack */
processmatch,
processmatchinfooffline,
NULL, /* processresult */
NULL, /* processresult info */
dfst);
}
seqit = gt_seqiterator_new(idxlocalioptions->queryfiles, err);
if (!seqit)
haserr = true;
if (!haserr)
{
gt_seqiterator_set_symbolmap(seqit, getencseqAlphabetsymbolmap(encseq));
for (showmatchinfo.queryunit = 0; /* Nothing */;
showmatchinfo.queryunit++)
{
retval = gt_seqiterator_next(seqit,
&query,
&querylen,
&desc,
err);
if (retval < 0)
{
haserr = true;
break;
}
if (retval == 0)
{
break;
}
printf("process sequence " Formatuint64_t " of length %lu\n",
PRINTuint64_tcast(showmatchinfo.queryunit),querylen);
if (idxlocalioptions->doonline || idxlocalioptions->docompare)
{
multiapplysmithwaterman(swdpresource,encseq,query,querylen);
}
if (!idxlocalioptions->doonline || idxlocalioptions->docompare)
{
indexbasedlocali(limdfsresources,
idxlocalioptions->matchscore,
idxlocalioptions->mismatchscore,
idxlocalioptions->gapstart,
idxlocalioptions->gapextend,
idxlocalioptions->threshold,
query,
querylen,
dfst);
}
if (idxlocalioptions->docompare)
{
checkandresetstorematch(showmatchinfo.queryunit,
&storeonline,&storeoffline);
}
gt_free(desc);
}
if (limdfsresources != NULL)
{
freeLimdfsresources(&limdfsresources,dfst);
}
if (swdpresource != NULL)
{
freeSWdpresource(swdpresource);
swdpresource = NULL;
}
gt_seqiterator_delete(seqit);
}
if (idxlocalioptions->docompare)
{
freestorematch(&storeonline);
freestorematch(&storeoffline);
}
}
if (genericindex == NULL)
{
gt_assert(encseq != NULL);
encodedsequence_free((Encodedsequence **) &encseq);
} else
{
genericindex_delete(genericindex);
}
freeverboseinfo(&verboseinfo);
return haserr ? -1 : 0;
}
