void BedIntersectPE::IntersectBamPE(string bamFile) {
// load the "B" bed file into a map so
// that we can easily compare "A" to it for overlaps
_bedB->loadBedFileIntoMap();
// open the BAM file
BamReader reader;
BamWriter writer;
reader.Open(bamFile);
// get header & reference information
string bamHeader = reader.GetHeaderText();
RefVector refs = reader.GetReferenceData();
// open a BAM output to stdout if we are writing BAM
if (_bamOutput == true) {
// set compression mode
BamWriter::CompressionMode compressionMode = BamWriter::Compressed;
if ( _isUncompressedBam ) compressionMode = BamWriter::Uncompressed;
writer.SetCompressionMode(compressionMode);
// open our BAM writer
writer.Open("stdout", bamHeader, refs);
}
// track the previous and current sequence
// names so that we can identify blocks of
// alignments for a given read ID.
string prevName, currName;
prevName = currName = "";
vector<BamAlignment> alignments; // vector of BAM alignments for a given ID in a BAM file.
alignments.reserve(100);
_bedA->bedType = 10; // it's a full BEDPE given it's BAM
// rip through the BAM file and convert each mapped entry to BEDPE
BamAlignment bam1, bam2;
while (reader.GetNextAlignment(bam1)) {
// the alignment must be paired
if (bam1.IsPaired() == true) {
// grab the second alignment for the pair.
reader.GetNextAlignment(bam2);
// require that the alignments are from the same query
if (bam1.Name == bam2.Name) {
ProcessBamBlock(bam1, bam2, refs, writer);
}
else {
cerr << "*****ERROR: -bedpe requires BAM to be sorted or grouped by query name. " << endl;
exit(1);
}
}
}
// close up
reader.Close();
if (_bamOutput == true) {
writer.Close();
}
}
void BedIntersectPE::IntersectBamPE(string bamFile) {
// load the "B" bed file into a map so
// that we can easily compare "A" to it for overlaps
_bedB->loadBedFileIntoMap();
// open the BAM file
BamReader reader;
BamWriter writer;
reader.Open(bamFile);
// get header & reference information
string bamHeader = reader.GetHeaderText();
RefVector refs = reader.GetReferenceData();
// open a BAM output to stdout if we are writing BAM
if (_bamOutput == true) {
// set compression mode
BamWriter::CompressionMode compressionMode = BamWriter::Compressed;
if ( _isUncompressedBam ) compressionMode = BamWriter::Uncompressed;
writer.SetCompressionMode(compressionMode);
// open our BAM writer
writer.Open("stdout", bamHeader, refs);
}
// track the previous and current sequence
// names so that we can identify blocks of
// alignments for a given read ID.
string prevName, currName;
prevName = currName = "";
vector<BamAlignment> alignments; // vector of BAM alignments for a given ID in a BAM file.
alignments.reserve(100);
_bedA->bedType = 10; // it's a full BEDPE given it's BAM
// rip through the BAM file and convert each mapped entry to BEDPE
BamAlignment bam1, bam2;
while (reader.GetNextAlignment(bam1)) {
reader.GetNextAlignment(bam2);
if (bam1.Name != bam2.Name) {
while (bam1.Name != bam2.Name)
{
if (bam1.IsPaired())
{
cerr << "*****WARNING: Query " << bam1.Name
<< " is marked as paired, but it's mate does not occur"
<< " next to it in your BAM file. Skipping. " << endl;
}
bam1 = bam2;
reader.GetNextAlignment(bam2);
}
}
else if (bam1.IsPaired() && bam1.IsPaired()) {
ProcessBamBlock(bam1, bam2, refs, writer);
}
}
// close up
reader.Close();
if (_bamOutput == true) {
writer.Close();
}
}
